Abstract
Raman scattering spectroscopies are important analytical tools in modern chemistry. Here, we describe the construction of the unamplified Raman scattering analysis (URSA) instrument: an inexpensive, open-table Raman spectrometer that uses a 532 nm diode-pumped laser as a light source. Upon application of this spectrometer to DNA extracted from strawberries with a soap solution and precipitated in isopropanol, two major Raman peaks with shifts of 740 and 2970 cm–1 were observed, consistent with previously published DNA Raman spectra. In this general article, we discuss the potential implementation of this work in an upper-level undergraduate laboratory, which would allow for hands-on exposure to both spectroscopic instrumental design and biochemical sample complexity.
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