Raman Imaging of the Distribution of Carotenoids in Haematococcus Pluvialis Under High Concentration of Sodium Chloride

Abstract

Carotenoids have important implications for maintaining normal cell activity, antioxidant and photosynthesis. Under some extremely hard conditions, the algae cells will produce a large number of carotenoids to resist the stress. The mechanism of algae cell emergency response has attracted a lot of attention. Here, Raman spectroscopy was applied to study the distribution of carotenoids in living algae cells under high concentration of sodium chloride. Multivariate analysis (MCR) was also used to resolve the corresponding Raman spectra of different carotenoids. By MCR analysis, we succeeded in distinguishing the distribution of different pigment cells. This method of analysis can be widely used in the study of mechanism of cellular stress response.