Abstract
Raman-enhanced spectroscopy (RESpect) probe, which enhances Raman spectroscopy technology through a portable fiber-optic device, characterizes tissues and cells by identifying molecular chemical composition showing distinct differences/similarities for potential tumor markers or diagnosis. In a feasibility study with the ultimate objective to translate the technology to the clinic, a panel of pediatric non-Hodgkin lymphoma tissues and non-malignant specimens had RS analyses compared between standard Raman spectroscopy microscope instrument and RESpect probe. Cryopreserved tissues were mounted on front-coated aluminum mirror slides and analyzed by standard Raman spectroscopy and RESpect probe. Principal Component Analysis revealed similarities between non-Hodgkin lymphoma subtypes but not follicular hyperplasia. Standard Raman spectroscopy and RESpect probe fingerprint comparisons demonstrated comparable primary peaks. Raman spectroscopic fingerprints and peaks of pediatric non-Hodgkin lymphoma subtypes and follicular hyperplasia provided novel avenues to pursue diagnostic approaches and identify potential new therapeutic targets. The information could inform new insights into molecular cellular pathogenesis. Translating Raman spectroscopy technology by using the RESpect probe as a potential point-of-care screening instrument has the potential to change the paradigm of screening for cancer as an initial step to determine when a definitive tissue biopsy would be necessary.
Highlights
And efficient diagnoses of solid tumors require histopathological evaluation of tissue specimens which remains the gold standard of differentiating malignant cells from normal cells
This study reports for the first time the use of Raman spectroscopy (RS) technology to discriminate childhood non-Hodgkin lymphoma (NHL) subtypes by standard RS instrumentation and by Raman-enhanced spectroscopy (RESpect) probe
While RS technology has previously characterized fingerprints of malignant tissues, characterizing RS fingerprints of childhood NHL subtypes has not been previously reported nor has the RESpect probe been tested on these types of cancers [2, 8, 18]
Summary
And efficient diagnoses of solid tumors require histopathological evaluation of tissue specimens which remains the gold standard of differentiating malignant cells from normal cells. The diagnostic workup of biopsy tissue focuses on morphological and molecular characteristics of individual cells to determine the malignant type which will lead to appropriate therapeutic action. This process has some limitations when timing is important to begin treatment. RS could pave the way to complement diagnostic paradigms in a timely fashion, in the pediatric setting where access to tissue can be challenging at times [3,4,5,6]
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