RAISING TECHNOLOGY INFLUENCES THE IMMUNITY AND BACTERIAL FLORA IN BOVINE

Abstract

Stressful factors specific to modern intensive raising systems and lacking in the traditional raising technologies are overcrowding, lack of sufficient vital space, social stress, etc., leading to immunesuppresion, pitfalls of productions, adaptative disorders, pathological behavior and disease (Man si col., 2002; Zahao, 2006). The study aimed to monitor the changes of digestive microflora in connection with systemic humoral immune effectors in lactating bovine raised under intensive (group I, n=12) and extensive (n=24)(household) technologies. The animals maintained in households were subdivided into two groups: II (n=12) kept in the same barn with horses and III (n=12) raised together with horses and swine. Blood samples and rectal swabs were taken from all the animals once. Microbiological examinations were carried out using selective media (MacConkey, XLD, Drigalsky) and API 20E galleries to identify the bacterial species. Zinc sulphate (24%o) and polyethylene glycol (4.2%) precipitation micro tests were used to quantify the total immuneglobulins (Vernes degrees) and circulating immune complexes’ (CIC, optical density units, ODU) levels in serum. The bacterial load was mainly Gram-negative, almost similar in groups I and II, the most important bacterial isolates being Klebsiella ornitholytica and E. coli. The highest variety of bacterial species was found in group III: Pseudomonas fluorexeus, Escherichia ferquassoni, Klebsiella ornithinolytica, Proteus mirabilis. The results obtained for the total Ig and CIC values of the experimental groups were presented in table 1. The highest, statistically non-significant, total Ig and CIC levels were observed in group II, inconsistent with the type of bacterial load