Abstract
We report the application of spin-echo 1H-NMR spectroscopy to the detection of raised plasma transaminase activity in rats treated with the nephrotoxic cephalosporin antibiotic cephaloridine (CPH). Spin-echo 1H-NMR analysis of lyophilized plasma, reconstituted in H 20 reveals a doublet at δ 1.48 for alanine. However when samples were reconstituted with 2H 20 we noted that in samples from CPH-treated rats (but not in control samples) there was a variable degree of appearance of a singlet at δ 1.47 together with a reduction in the doublet at δ 1.48. We suggest that this is due to the release of transaminases from damaged tissue which, via a reversible conversion of alanine to pyruvate, causes selective deuteration of alanine at the α-hydrogen (α-CH) position. This observation suggests that these 1H-NMR spectral patterns are dependent on the level of plasma transaminases and this may provide a novel indicator of tissue damage.
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