Rahnella perminowiae sp. nov., Rahnella bonaserana sp. nov., Rahnella rivi sp. nov. and Rahnella ecdela sp. nov., isolated from diverse environmental sources, and emended description of the genus Rahnella.

Carrie Brady,Stephanus Venter,May Bente Brurberg,Sandra Denman,Bridget Crampton,Dawn Arnold,Jo Ann Asselin,Steven Beer

doi:10.1099/ijsem.0.005190

Abstract

Bacteria isolated from onion bulbs suffering from bacterial decay in the United States and Norway were previously shown to belong to the genus Rahnella based on partial housekeeping gene sequences and/or fatty acid analysis. However, many strains could not be assigned to any existing Rahnella species. Additionally, strains isolated from creek water and oak as well as a strain with bioremediation properties were assigned to Rahnella based on partial housekeeping gene sequences. The taxonomic status of these 21 strains was investigated using multilocus sequence analysis, whole genome analyses, phenotypic assays and fatty acid analysis. Phylogenetic and phylogenomic analyses separated the strains into five clusters, one of which corresponded to Rahnella aceris. The remaining four clusters could be differentiated both genotypically and phenotypically from each other and existing Rahnella species. Based on these results, we propose the description of four novel species: Rahnella perminowiae sp. nov. (type strain SL6T=LMG 32257T=DSM 112609T), Rahnella bonaserana sp. nov. (H11bT=LMG 32256T=DSM 112610T), Rahnella rivi sp. nov. (FC061912-KT=LMG 32259T=DSM 112611T) and Rahnella ecdela sp. nov. (FRB 231T=LMG 32255T=DSM 112612T).

Highlights

Rahnella species grew well in nutrient broth supplemented with up to 6 % NaCl, while weak growth was observed at 7 % NaCl
Strains grow well at pH 5 - 8 and in broth supplemented with NaCl up to 6 %
Rahnella aquatilis (n = 1), 6 = Rahnella aceris (n = 4, type strain not included), 7 = Rahnella bruchi (n=3), 8 = Rahnella contaminans (n = 1), 9 = Rahnella inusitata (n=3), = Rahnella laticis (n = 2), = Rahnella variigena (n=4), = Rahnella victoriana (n=4), = Rahnella woolbedingensis (n=3)

Summary

Conflicts of interest and disclaimers

The authors declare that there are no conflicts of interest. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S.

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Methods

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