RAFT Method for Preparation of Molecularly Imprinted Polymer–SBA15 Nanocomposite as a Pipette Tip Solid-Phase Microextraction Adsorbent

Abstract

Methoxyfenozide-selective surface molecularly imprinted polymer (MIP) sorbent was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization based on support matrix of SBA-15. The size, morphology, composition, and properties of the prepared nanocomposite have also been characterized and determined using scanning electron microscopy (SEM) and Fourier-transform- infrared (FT-IR) spectroscopy. A miniaturized solid-phase extraction procedure has been developed for ultra-trace determination of methoxyfenozide adsorbed on polymethacrylic acid-functionalized Santa Barbara Amorphous type material (SBA-15), which is packed into a pipette- tip (PT). The PT-SPE was assembled by packing 2.0 mg of nanocomposite of MIP as sorbent into a 100 μL pipette tip. The method was optimized for several important extraction parameters such as sample volume, pH, amount of sorbent, and eluent solvent. The results determine that the prepared adsorbent has a high extraction power. High sensitivity, non-use of organic solvent, high selectivity, and low detection limits are the unique advantages of the proposed method.