Abstract

The effects of diets containing raffinose with or without Lactobacillus acidophilus plus Streptococcus thermophilus were investigated in rats. Raffinose induced a decrease in body weight. Caecal weight was increased by feeding raffinose plus lactic acid bacteria (LAB). Raffinose ingestion increased the caecal concentration of lactobacilli but decreased numbers of streptococci. Concurrent administration of raffinose plus LAB decreased the staphylococci and clostridia numbers. Caecal pH was significantly reduced by feeding raffinose or raffinose plus LAB. Adding LAB to a raffinose containing diet induced a decrease in azo reductase activity of the caecal contents. Raffinose, with or without LAB, increased total volatile fatty acids (VFA) production. Caecal butyrate was higher with the raffinose and LAB diet than with raffinose alone or control diets. Although total triglycerides in plasma was lowered by raffinose, this sugar did not affect concentrations of plasma cholesterol or high density lipoproteins (HDL). However, a significant increase in the HDL cholesterol was observed in response to a concurrent administration of raffinose plus LAB. Copyright © 1996 Elsevier Science Inc.

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