Abstract
The extracts of the three official Rheum spec., Rheum palmatum, Rheum tanguticum and Rheum officinale possess a very similar qualitative composition of anthraquinone glycosides and their free aglycones. They can be hardly distinguished from each other by TLC alone. The anthraquinone aglycones show quantitatively also a very similar fingerprint pattern. The adulterations, Rheum rhaponticum or Rheum undulatum, characterized by a high content of the phytoestrogenic stilbenes (rhaponticin, isorhaponticin and desoxyrhaponticin) can be easily detected by TLC due to two distinct blue fluorescent bands at Rf-value around 0.5. The corresponding graphs of the HPLC-fingerprints of the three official Rheum spec. show also a very similar qualitative peak pattern, without the possibility of a clear discrimination, whereas the adulterations of Rheum rhaponticum or Rheum undulatum can be again easily detected due to three significant peaks, not present in other Rheum species.
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