Abstract

 
 
 
 Purpose: To investigate the radiosensitizing effect of polysaccharide from Schisandrae Chinensis fruit (SCFP) on ovarian cancer SKOV3 cell line.
 Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate SKOV3 cell viability. Caspase-3, caspase-9, Bax, and Bcl-2 levels in SKOV3 cells were assessed by enzyme-linked immunosorbent assay (ELISA). Furthermore, mRNA expression of caspase-3, caspase-9, Bax and Bcl-2 in SKOV3 cells were determined using quantitative real-time polymerase chain reaction (RT-qPCR) assay.
 Results: β-Irradiation (10 Gy) did not show obvious inhibitory effects on SKOV3 cell proliferation. However, SCFP at a dose of 1.25, 2.5 and 5 mg/mL significantly enhanced the radiosensitivity of SKOV3 cells to β-irradiation (p < 0.01). Furthermore, SCFP at concentrations of 1.25, 2.5 and 5 mg/mL significantly increased the levels of caspase-3, caspase-9 and Bax in SKOV3 cells, but decreased that of Bcl-2 (p < 0.01). In addition, SCFP combined with β-irradiation up-regulated mRNA expression of caspase-3, caspase-9 and Bax (p < 0.01), but down-regulated that of Bcl-2 in SKOV3 cells (p < 0.01).
 Conclusion: SCFP significantly enhances the sensitivity of nasopharyngeal carcinoma SKOV3 cells to β-irradiation. The possible mechanism of sensitization might be correlated to the induction of mitochondria-mediated apoptosis. This research provides a scientific basis for the development of SCFP for the treatment of ovarian cancer.
 
 
 
Highlights
Ovarian cancer has been reported to be the fifth leading cause of oncological-related death in women in the last four decades
We found that SCFP treatment at doses of 10 (p < 0.05), 20 (p < 0.01), and 40 mg/mL (p < 0.01) exerted significant cytotoxicity on SKOV3 cells (Figure 1)
We found that 10 Gy β-irradiation did not have significant inhibitory effects on the proliferation of SKOV3 cells (p > 0.05)
Summary
Ovarian cancer has been reported to be the fifth leading cause of oncological-related death in women in the last four decades. It has been reported that polysaccharides in SCF (SCFP) inhibit the proliferation of ovarian cancer SKOV3 cells [6], the mechanism is unclear. The present study aimed to analyze the radiosensitizing effect of SCFP on ovarian cancer SKOV3 cells and determine its possible underlying mechanism. Ovarian cancer SKOV3 cell line was obtained from Shanghai SuRan Biotechnology Co., Ltd. SKOV3 cells were cultured in RPMI1640 supplemented with 10 % FBS and grown in an incubator with 5 % CO2 at 37 °C. SKOV3 cells were exposed to β-irradiation in addition to the SCFP treatment. Effect of SCFP on the protein expression of caspase-3, caspase-9, Bax, and Bcl-2 in SKOV3 cells. To explore the possible mechanism of the enhanced radiosensitivity of SCFP, proteins related to the apoptosis pathway, including caspase-3, caspase-9, Bax, and Bcl-2 were detected in SKOV3 cells using an ELISA assay
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