Abstract

The in vitro radiosensitivity of one murine melanoma cell line (Cloudman S91 CCL 53.1) and three human melanoma cell strains (C8146C, C8161, and R83-4) were studied. Cells were irradiated by single dose X-rays and plated either in agar or on plastic. The survival curves were fitted by the single-hit multitarget, two-hit multitarget, and quadratic models. Multiple comparisons of the residual sum of squares suggested that the two-hit model was clearly inferior to the single-hit and quadratic models. No statistically significant difference was suggested for either the single-hit or quadratic models. Furthermore, on examination of the differences in correlations between the observed and predicted values, the residual plots (observed minus predicted over dose) failed to suggest a clear advantage of either the single-hit multitarget or the quadratic models. Either model could be recommended for analysis of in vitro radiation data.

Highlights

  • The human tumor clonogenic assay described by Hamburger and Salmon (1977) has been utilized by investigators studying the efficacy of therapeutic agents for several tumor types

  • Interlaboratory differences between cloningsystems, radiation protocol, and statistical analysis used to analyze survival curves have hindered the acquisition of reproducible data to delineate radiation response of human melanoma (Barranco et al, 1971; Courdi et al, 1981; Fbfstad and Brustad, 1981,1983;Selby and Courtenay, 1982;Smith et al, 1978; Weichselbaum et al, 1980; Weininger e t al., 1978)

  • We determined the residual sum of squares and the correlation of the observed with predicted values of the data using the bly for the melanoma cells that we studied (Yohem et al, 1988) which suggested the possibility that differences in radiation sensitivity may be due to differences in the intrinsic radiosensitivity of the cells as well as the assay and mathematical model used to describe radiosensitivity

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Summary

Introduction

The human tumor clonogenic assay described by Hamburger and Salmon (1977) has been utilized by investigators studying the efficacy of therapeutic agents for several tumor types. The study of radiation survival of cells grown on plastic began in 1956 with the classic experiments of Puck and Marcus. Together both of these assays have been used in studying the response of tumor cells to a wide variety of agents. Our results were similar to those of Fertil et al We found that the single-hit multitarget model fit the radiation data. We conclude that either the single-hit multitarget or the quadratic models can be used for analysis of in vitro melanoma radiation data

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