Abstract
Objective To investigate the radioseusitivity of brain cancer stem cells of different conditions isolated from malignant glioma cell line U251 in vitro. Methods The brain cancer stem cells in U251 or the brain cancer stem cells isolated from U251 were irradiated by 60 Co γ-rays. TUNEL and Annexin-FITC were employed to detect the apoptosis. The brain cancer stem cells were subcutaneously transplanted to nude mouse. Flow cytometry was used to detect cell cycle. Results The brain cancer stem cells isolated from malignant glioma cell line U251 were in active cell cycle and sensitive to 60Co γ-rays. Thed apoptotic cells were increased obviously after irradiation. After subcutaneously transplanted to made mouse, there was no tumor appear. However, the brain cancer stem cells existed in U251 were in G0-G1 and resisted to 60Co γ-rays. They differentiated into the parent glioma type after traqnsplantation. Conclusions The brain cancer stem cells existed in the malignant glioma cell line is resisted to irradiation, and this phenomenon may explain the glioma relapse in situ after radiation therapy. Key words: Glioma; Cancer stem cells; Magnetic cell sorting; Radiosensitivity
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