Abstract
The purpose of this study was to investigate the protective effect of low dose radiation on TCE induced oxidative damage in rats. The oxidative damage of both liver and kidney was assessed by serum alkaline phosphatase (ALP), Gamma Glutamyl Trans-Peptidase (GGTP), Alanine and Aspartate Amino Transferase (ALT & AST) activities in addition concentrations of cholesterol, high density lipoprotein-cholesterol (HDL-c), low density lipoprotein-cholesterol (LDL-c), Triacyglycerols (TGs), urea and creatinine were analyzed. Liver lipid peroxidation (MDA), Nitric Oxide (NO), reduced glutathione (GSH) levels, and activities of both Super-Oxide Dismutase (SOD) and Glutathione Peroxidase (GSH-Px) were measured. Results: TCE administration increase serum ALP, GGTP, ALT, AST activities, cholesterol, triacylglycerols, LDL-c, urea and creatinine concentrations, besides liver MDA and NO, whereas it decreased SOD, GSH-Px activities, GSH level in liver, HDL-c in serum. Low dose of gamma rays (0.5 Gy) exposure significantly improved lipid peroxidation and oxidative injury induced by TCE. Conclusion: The study indicates that treatment with low dose of gamma rays ameliorate harmful effects induced by TCE taking in consideration the effect of gamma radiation as a stimulant of radical detoxification.
Highlights
The concept of radiation hormesis is usually applied to physiological benefits from low radiation dose ranging from 1 - 50 cGy
This study was carried to evaluate the liver and kidney function tests, lipid profile in serum and some enzymatic and non-enzymatic antioxidant such as Super-Oxide Dismutase (SOD), GSH-Px activities as well as reduced GSH, MDA and Nitric Oxide (NO) concentration in liver homogenate, besides certain trace elements (Fe, Cu, Zn and Mn) in liver after radiation treatment to improve the toxic effects of trichloroethylene in male albino rats
The present study revealed that the injection of TCE (500 mg/kg b.w) resulted in elevation in levels of ALT, AST, Gamma Glutamyl Trans-Peptidase (GGTP), and alkaline phosphatase (ALP) on the 1st and 3rd days post TCE injection
Summary
The concept of radiation hormesis is usually applied to physiological benefits from low radiation dose ranging from 1 - 50 cGy. According to Manno, Yamoaka, Kojima and their colleagues and Feinendegen, pre-irradiated animals with low dose may enhance the activities of SOD, GSH-Px [2,6,7,8], while Nomura and Yomaoka, suggested that low dose radiation reduced oxidative damage induced by CCl4 in mouse liver, this by increasing the endogenous antioxidant in animal tissues [9]. Suppression in oxidative injury by endogenous and exogenous antioxidant has proven effective Kojima and his colleagues found that liver GSH level in male mice increased 2 hr after irradiation with 60 cGy of gamma rays, reduced a maximum at around 4 hr and returned almost to the control level by 12 hr [10]
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