Radioprotective effect of diethylcarbamazine on radiation-induced acute lung injury and oxidativestress in mice.

Abstract

The present study was designed to evaluate the radioprotective effect of diethylcarbamazine (DEC) against oxidative stress and acute lung injury induced by total body radiation (TBI) in mice. For study the optimum dose for radiation protection of DEC, mice were administrated with three dose of DEC (10, 50 and 100mg/kg), once daily for eight consecutive days. Animals were exposed whole body to 5Gy X-radiation on the 9day. The radioprotective potential of DEC in lung tissues was assessed using oxidative stress examinations at 24h after TBI and histopathological assay also was analyzed one week after TBI. Results from biochemical analyses demonstrated increased malonyldialdehyde (MDA), nitric oxide (NO) and protein carbonyl (PC) levels of lung tissues in only irradiated group. Histopathologic findings also showed an increase in the number of inflammatory cells and the acute lung injury in this group. DEC pretreatment significantly mitigated the oxidative stress biomarkers as well as histological damages in irradiated mice. The favorable radioprotective effect against lungs injury was observed at a dose of 10mg/kg of DEC in mice as compared with two other doses (50 and 100mg/kg). The data of this study showed that DEC at a dose of 10mg/kg with having antioxidant and anti-inflammatory properties can be used as a therapeutic candidate for protecting the lung from radiation-induced damage.