Radiolytically degraded sodium alginate enhances plant growth, physiological activities and alkaloids production in Catharanthus roseus L.

Abstract

Catharanthus roseus (L.) G. Don (Family Apocynaceae) is a medicinal plant that produces indole alkaloids used in cancer chemotherapy. The anticancerous alkaloids, viz. vinblastine and vincristine, are mainly present in the leaves of C. roseus. High demand and low yield of these alkaloids in the plant has led to explore the alternative means for their production. Gamma irradiated sodium alginate (ISA) has proved as a plant growth promoting substance for various medicinal and agricultural crops. A pot culture experiment was carried out to explore the effect of ISA on plant growth, physiological activities and production of anticancer alkaloids (vinblastine and vincristine) in C. roseus at 120 and 150 days after planting (DAP). Foliar application of ISA (0, 20, 40, 60, 80 and 100 mg L−1) significantly improved the performance of C. roseus. 80 mg L−1 of ISA enhanced the leaf-yield by 25.3 and 30.2% and the herbage-yield by 29.4 and 34.4% at 120 and 150 DAP, respectively, as compared to the control. The spray treatment of ISA at 80 mg L−1 improved the yield of vinblastine by 66.7 and 71.4% and of vincristine by 67.6 and 75.6% at 120 and 150 DAP, respectively, in comparison to the control. As compared to control, the application of ISA at 80 mg L−1 resulted in the maximum swell in the content and yield of vindoline, increasing them by 18.9 and 20.8% and by 81.8 and 87.2% at 120 and 150 DAP, respectively.