Abstract
Various modifications of the radioisotope assay of vitamin B 12 in serum were examined. The technique of extracting vitamin B 12 from the serum strongly influenced the end results, as did addition of KCN. A modification was chosen in which extraction was achieved by deproteinization during autoclaving. To ensure that all the vitamin B 12, labelled and unlabelled, was in the same form (cyano-form), KCN was added. This modified isotope method shows satisfactory correlation with a microbiological assay using Lactobacillus leichmannii as test organism. However, in sera from patients receiving chlorpromazine, the isotope method gave normal values, while the microbiological assay disclosed diminished levels. Applying the proposed method to clinical material, we found diminished vitamin B 12 levels in 8 cases of pernicious anaemia.
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