Abstract
Biodistribution and role of extracellular vesicles (EVs) are still largely unknown. Reliable tracking methods for EVs are needed. In this study, nuclear imaging using radioiodine were developed and applied for tracking EVs derived from cell lines. EVs were obtained from supernatant of thyroid cancer cell (Cal62) and natural killer cells (NK92-MI) using sequential ultracentrifuges. Sulfosuccinimidyl-3-(4-hydroxypheynyl) propionate were labeled to membrane of Cal62 and NK92-MI cell derived EVs, then the EVs were labeled with radioiodine (I-131 and I-125) using pre-coated iodination tubes (RI-EVs). In vivo gamma camera images were obtained after intravenous injection of the RI-EVs, and ex vivo biodistribution study was also performed. EVs were labeled with radioiodine and radiochemical purity of the RI-EV was more than 98%. Results of nanoparticle tracking analysis and electron microscopy showed that there was no significant difference in EVs before and after the radioiodine labeling. After intravenous injection of RI-EVs to mice, gamma camera imaging well visualized the real-time biodistribution of the RI-EVs. RI-EVs were mainly visualized at liver, spleen, and lung. Nuclear imaging system of EVs derived from thyroid cancer and NK cells using radioiodine labeling of the EVs was established. Thus, this system might be helpful for in vivo tracking of EVs.
Highlights
Creative Future Talents, School of Medicine, Kyungpook National University, Daegu, Republic of Korea. *email: Scientific Reports | (2021) 11:5041
In vivo imaging of the radiolabeled Extracellular vesicles (EVs) using gamma camera was performed in normal mice, and corresponding results were compared between in vivo imaging and biodistribution study
After purification of EVs, I-131 was successfully labeled to EVs (Fig. 1)
Summary
Creative Future Talents, School of Medicine, Kyungpook National University, Daegu, Republic of Korea. *email: Scientific Reports | (2021) 11:5041. Creative Future Talents, School of Medicine, Kyungpook National University, Daegu, Republic of Korea. Has been widely used for in vivo tracking of cells and other biomaterials[13,14,15], only several reports were published for tracking EVs using the nuclear imaging[16,17,18,19]. The labeling method of radioiodine to EVs was established, and characterizations of EVs before and after the radioiodine labeling were performed. In vivo imaging of the radiolabeled EVs using gamma camera was performed in normal mice, and corresponding results were compared between in vivo imaging and biodistribution study
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