Abstract
Radioactive iodide was covalently linked to the cell-surface proteins of an established insect cell line, by the surface-radioiodination method of Haustein (1975) developed for in vitro, cultured neoplastic lymphocyte cell lines. Maximally, 2.4 × 10 5 atoms of [ 125I]-iodide/cell could be covalently bound, corresponding to a 20% uptake of label, without any deleterious effect on cell viability. Although the amount of macromolecular-bound [ 125I]-iodide was time-dependent, it was not in a linear fashion. There is an indication of a 72 hr cycle of binding activity. Protein distribution patterns·showed quantitative not qualitative changes for the cells, with time, when resolved by SDS gel electrophoresis. Comparison of two insect cell sublines at 72 hr subculture, revealed quantitative rather than qualitative changes in the protein profiles.
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