Abstract
The use of immunosorbents for radioimmunoassays of proteins and polypeptides has considerably simplified these methods. The immunosorbents were prepared by chemical coupling of antibodies to derivatives of insoluble dextran (Sephadex). Four coupling methods were compared: three cyanogen halide methods and one isothiocyanato-method. It was concluded that immunosorbents prepared by CNBr-activation of Sephadex are stable for at least three months, have a high antigen binding capacity, have a very small tendency for nonspecific adsorption, and are easiest to prepare in comparison with the other immunosorbents investigated. This method is recommended for the preparation of immunosorbents to be used in the radioimmunosorbent assay system.
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