Abstract
Various methods of detecting specific IgG4 antibodies have been described. These include the radioallergosorbent test (RAST), 1-3 polystyrene tube radioimmunoassay (RIA), 4 enzyme-linked immunosorbent assay (ELISA), s-7 quantitative immunofluorescence defined antigen substrate spheres system (DASS), 3 red cell linked antigen-antiglobulin reaction (RCLAAR), s microtiter solid phase radioimmunoassay (MSPRIA), 9 and histamine release. 1°'1~ RAST, polystyrene tube RIA, and MSPRIA are all radioimmunoassays, in which antigen-specific antibodies in serum samples bind to solid-phase-bound antigens and can thus be detected by radiolabeled subclass-specific reagents. In the RAST method, antigens are chemically coupled to cellulose or Sepharose 4B using cyanogen bromide. Serum samples are allowed to incubate at room temperature with these antigen-coupled cellulose disks or Sepharose, usually for 24 hr. The disks or Sepharose are then washed extensively and developed with radiolabeled (~2sI) human-IgG4-specific affinity column purified antibodies. The results are usually expressed as percentages of added radioactivity.
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