Abstract
Radioimmunoassays for urinary gonadotrophins and insulin were developed utilizing second-antibody immunosorbents to separate bound and free labelled hormone. By using these immunosorbents the versatility of the double-antibody procedure was combined with the ease and speed of handling of the solid-phase methods. The immunosorbents were prepared by coupling antibodies raised in sheep against rabbit or guinea pig γ-globulins (a-RGG or a-GPGG, respectively) to agarose and cellulose. Solid-phase radioimmunoassays for rabbit and guinea pig γ-globulin (RGG and GPGG, respectively) were used for studying the properties of these immunosorbents. The properties and applicability of a-RGG-immunosorbents were investigated in radioimmunoassays for HCG/LH and FSH. The effect of different incubation times, of varying the concentration of the first antibody, and of second-antibody immunosorbents on the percentage of labelled hormone precipitated was studied. The applicability of a-GPGG-immunosorbents was investigated in an insulin assay system. The specificity of the assays, which utilize this bound/free separation method, is described.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
