Abstract
□ A radioimmunoassay was developed for the measurement in human serum and urine of erythro-9-(2-hydroxy-3-nonyl)-hypoxanthine. Antisera were produced in rabbits by immunization with an erythro-9-(2-hydroxy-3-nonyl)-hypoxanthine hemisuccinate–bovine serum albumin conjugate. The competitive antigen was erythro-9-(2hydroxy-3-nonyl)-hypoxanthine labeled with carbon-14 on the purine ring. Cross-reactivities were measured against three metabolites and the naturally occurring purine bases inosine and hypoxanthine. Sensitivity of the method was 1ng/ml in serum and 10ng/ml in urine. Precision at clinical levels was ±15% in serum at 2ng/ml and ±3% in urine at 200ng/ml.
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