Abstract
A double-antibody radioimmunoassay (RIA) to acute-phase α 2-macroglobulin was developed for the quantitation of this large macromolecule in physiological fluids. The primary receptor for the RIA was a monospecific antisermn to purified acute-phase α 2-macroglobulin which produced a high titre (7.5.10 6) antibody with a strong affinity for rat acute-phase α 2.macroglobulin ( Ka= 1.24.10 11) as measored by Scatchard analysis. The validity of the assay was confirmed by specificity for rat α 2-macroglobulin measured in various physiological fluids as assessed by parallel dose-response curves; and accuracy, measured by the analytical recovery of α 2-macroglobulin by the RIA in serum (104±7%) and buffer (103±7%), and the correlation ( R=0.999) of measurements of acute-phase α 2-macroglobulin-containing samples measured in serum and buffer. Reference acute-phase serum measured by this RIA and by rocket immunoelectrophoresis were 98.6% in agreement. Radioimmunoassay sensitivity was estimated at less than 1.0 ng α 2-macroglobulin/ml, measured over a range of 0∗160 ng. Precision was assessed by intraassay (2.99±0.97%) and interassay (8.76±2.64%) variation. Evaluation confirmed that quantitation of rat acute-phase α 2-macroglobulin by this RIA met the criteria of sensitivity, validity and precision.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular Enzymology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.