Radioimmunoassay of prostaglandins

Abstract

The earlier radioimmunoassays were mainly intended for the measurement of prostaglandins of the E-F-A or B type in blood plasma/serum or urine. Many recent studies, however, explain the use of radioimmunoassay to measure the prostaglandin content of tissues, and many other studies are concerned with the prostaglandin production in a single cell type, or in a few cell types, rather than the whole tissue. To date, however, by far the greatest number of quantitative prostaglandin studies have been carried out on blood plasma or serum, while assay for primary prostaglandins are now fairly seldom applied to the peripheral circulation, unless it is to study the prostaglandin production in vivo. It has been proposed that prostglandins of the A type are circulating hormones in contrast to other prostglandins, and a number of laboratories have developed quantitative methods for the measurements of PGA compounds. The sensitivity and specificity of the prostaglandins radioimmunoassays have increased considerably in later years through the use of labelled ligands of better quality; on the other hand, the accuracy of many radioimmunoassays seems to be very low when they are applied to biologic materials.