Abstract
A reliable, direct radioimmunoassay of plasma angiotensin II (PAII) is described. No cross-reactivity of human renin, sheep renin substrate, saralasin acetate and angiotensin I with the anti-angiotensin II antiserum was found. However, des- 1Asp-angiotensin II has a stronger binding affinity for the antiserum than angiotensin II. A significant correlation was found between PAII determined in plasma samples without and with extraction on a Dowex 50W-X 2 resin. The combination of EDTA and o-phenanthroline has been found to be a very efficient angiotensinase inhibitor in plasma and is of equal potency as EDTA and diisopropylfluorophosphate in inhibiting the angiotensinase and converting enzyme activity. A significant correlation was found between PAII measurements performed in plasma samples in the presence of both inhibitor solutions. The intra-assay variations were checked by multiple analysis of a plasma pool and the inter-assay variations by measuring the angiotensin II concentration in different plasma samples on two occasions. The mean PAII in 10 healthy, male subjects on an ad libitum diet was 25.1 ± 13.5 (S.D.) pg/ml (range: 10.1 to 51.4). PAII correlated also better with plasma renin activity (active renin) than with plasma renin concentrations, measured after acidification of plasma (total renin).
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