Abstract
Radioimmunoassay method for the determination of ginsenoside Rg1, one of the major saponins of Panax ginseng root, was established. The side chain of ginsenoside Rg1 was modified and coupled to BSA. 125I-Labelled compound of ginsenoside Rg1 was prepared from a ginsenoside Rg1-tyramine conjugate. Immunization of rabbits with the hapten-BSA conjugate gave antiserum possessing high affinity and specificity toward ginsenoside Rg1. Ginsenoside Rg1 can be determined by the established radioimmunoassay method over a range of 250 pg-10 ng per tube.
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