Abstract
A sensitive and specific radioimmunoassay has been developed for luteinizing hormone (LH) in serum from rhesus monkeys using a unique anti-ovine LH serum (GDN-15) and ovine LH for radioiodination. It did not appear that high levels of follicle-stimulating hormone (FSH) or thyroid-stimulating hormone (TSH) influenced the measurement of LH in this assay. The good agreement between the estimates of LH by radioimmunoassay and by bioassay for a crude pituitary extract suggested that other pituitary factors did not influence the measurement of LH. Exogenous LH could be completely and quantitatively recovered when added to serum. Levels of LH increased in serum following castration of 2 females and decreased to nondetectable levels following 10 days of estrogen treatment of these same animals. There was no detectable activity in serum from some hypophysectomized females, while others had varying amounts of inhibiting material which behaved similarly to highly purified rhesus LH as judged by immunologic, electrophoretic and gel filtration analysis, but this material remains to be fully characterized. Both the general patterns and the absolute levels of LH in serum from normally cycling females were very similar to those reported previously. (Endocrinology88:1327,1971)
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