Abstract
The development of the radioimmunoassay (RIA) for ecdysteroids has permitted the sequential measurement of circulating ecdysteroids on individual organisms. In addition, used in conjunction with various chromatographic techniques, it has assisted in the identification of ecdysteroids secreted by molting glands. The sensitivity of the assay has also permitted the monitoring of the rate of secretion of ecdysteroids by molting glands exposed to various in vitro conditions. This latter approach will be of great utility in determining the nature and effect of molt regulators (inhibitors) present in other tissues.
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