Abstract
Rabbit and chicken IgG anti-fluorescyl antibodies were fractionated into subpopulations differing in their average intrinsic association constants. Each subpopulation was analyzed qualitatively and quantitatively for its carbohydrate content by the radiochromatograhic assay. Results show that each affinity subpopulation derived from an individual animal varied significantly in carbohydrate composition. However, results from different species and/or individuals were not directly comparable. The data were discussed in terms of quantitative and qualitative differences in the carbohydrate moieties of each subpopulation.
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