Abstract
Reactions involving lipase-catalyzed interesterification of lipids, which are of commendable interest in biotechnology, have been monitored and assayed by radiochemical methods using 14C-labeled substrates. Medium chain (C12 plus Cu) triacylglycerols were reacted in the presence of an immobilized lipase from Mucor miehei and hexane at 45 °C with methyl [1-14C]oleate. [1-14C]oleic acid, [carboxyl-14C]trioleoylglycerol. [1-14C)octadecenyl alcohol, and [U-14C)glycerol, each of known specific activity. The reactions were monitored and the rate of interesterification determined by radio thin layer chromatography from the incorporation of radioactivity into acyl moieties of triacylglycerols (from methyl oleate, oleic acid, and trioleoylglycerol), alkyl moieties of wax esters (from octadecenyl alcohol), and into glycerol backbone of monoacylglycerols and diacylglycerols (from glycerol).
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