Abstract
This technique gives accurate, reproducible results in a short time. It is particularly suitable for studying alanine, aspartic acid, glutamic acid, isoleucine, leucine, methionine, phenylalanine, selenomethionine, serine, tyrosine, and valine. A major factor contributing to the accuracy and the precision of the method is the statistical accuracy when scanning. The value obtained for the “blank” will be crucial to the accuracy of the determination. When scanning, the same portion of the tlc plate is examined to determine the α-keto acid and to determine the value of the “blank” to be subtracted to give the true d -isomer value. Tritium samples were not determined with the same accuracy or precision as the other isotopes due to the inferior single detector scanner in use. The method cannot be used for tritiated samples labeled in the 2-position due to the loss of activity.
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