Radioautographic solubility studies of I131 and P32 in frozen-dehydrated tissues.

Abstract

A previous report(1) described a method for preparation of radioautographs of animal tissues without isotope loss.∗ This work demonstrated the ease of extraction of P32 from frozen-dried liver sections when they come into contact with water. This result agrees with chemical studies of P32 distribution in tissues which have shown that a major part of the P32 should be present in water soluble form(2). The present report describes results obtained when frozen-dehydrated tissue sections containing radioisotopes are exposed to extraction by a variety of reagents, both aqueous and nonaqueous. Radioautographic exposures were made with the extracted tissue sections and also with non-extracted control sections, as will be described. The results were evaluated by comparing the photographic densities and pattern of localization of radioautographs obtained from analogous tissue sections subjected to various types of extraction with those of the control sections. It is an application of the radio-autographic method t...