Abstract
Irradiations of plasmid DNA by both X-rays and UV light in the presence and absence of compounds containing platinum and bromine atoms were performed in order to asses the sensitization potential of these compounds. Plasmid DNA pBR322 was incubated with platinum (II) bromide, hydrogen hexabromoplatinate (IV), hydrogen hexahydroxyplatinate (IV) and sodium hexahydroxyplatinate (IV). Incubation was followed by X-ray or UV irradiations. It was found that amongst the sensitizers tested, during irradiations carried out in the presence of platinum (II) bromide, the highest levels of double strand breaks formation upon X-ray treatment were recorded. In contrast much less damage was induced by UV light. Data presented here suggests that this compound may be a promising radiosensitizer for cancer treatment.
Highlights
A comprehensive understanding of the mechanisms by which high- and low-energy radiation interacts with cellular components, DNA in particular, underlays advances in modern radiotherapy
In the present work we investigate three other platinum (IV) compounds as possible candidates for therapeutic drugs and cross examine them against PtBr2 (Fig. 1)
DNA mixed with Na2Pt(OH)6, irradiated with UV light at 215, 260, 320 and 370 nm
Summary
A comprehensive understanding of the mechanisms by which high- and low-energy radiation interacts with cellular components, DNA in particular, underlays advances in modern radiotherapy. For many years attempts were made to increase the levels of lethal cellular damage in a controlled manner using various types of drugs, that apart from chemical damage, may act as radiosensitizers. Such concomitant treatment has been shown to be beneficial in the treatment of neck, lung, pancreas or stomach cancers [1]. Recent research has highlighted that low-energy electrons, which are the most abundant species created during high energy irradiations, may be as efficient in causing damage to DNA [2], as the incident radiation itself Such secondary-particle damage can be initiated by any type of ionizing radiation, such as X-rays or γ-rays. It has been shown that such compounds coordinate themselves to nitrogen atoms of purines in DNA
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