Radical Scavenging and Reducing Power of Salvia mirzayanii Subfractions

Mahmood Reza Moein,Soheila Moein,Saeid Ahmadizadeh

doi:10.3390/molecules13112804

Abstract

In this research, the radical scavenging activity and reducing power of the ethyl acetate fraction and subfractions of Salvia mirzayanii (SM) have been investigated. The plant material was initially extracted with ethanol. The fractionation was carried out using liquid-liquid extraction, then the ethyl acetate fraction, which showed the greatest antioxidant activity, was selected. This fraction was submitted to column chromatography on a Sephadex LH 20 column eluted with pure MeOH to obtain subfractions A-G. No significant differences exist between the IC50 of Salvia mirzayanii ethyl acetate subfraction C (IC50 = 37.9 ± 0.85), F (IC50 = 40.05 ± 1.4) and quercetin (38.84 ± 0.86), (P > 0.05), indicating that the radical scavenging capacity of these two subfractions and quercetin (antioxidant standard) were similar. The reducing power of the ethyl acetate fraction was less than that of all subfractions, except for subfraction A. The greatest amount of phenolic compounds was found in subfraction E (55.23 ± 4.2) and the lowest in subfraction F (5.23 ± 0.18). The greatest total flavonoid content was established in subfraction D (1.84 ± 0.01) and the lowest was in subfraction A (0.108 ± 0.007).

Highlights

It is well known that reactive oxygen species (ROS) such as O2, H2O2(hydrogen peroxide), and OH are closely involved in various human diseases such as Alzheimer's disease, aging, cancer, inflammation, rheumatoid arthritis and atherosclerosis [1,2,3]
A great number of natural medicinal plants have been tested for their antioxidant activities and results have shown that the raw extracts or isolated pure compounds from them were more effective antioxidants in vitro than butylated hydroxytoluene (BHT) or vitamin E [7,8,9], so, medicinal plants can be a potential source of natural antioxidants [10]
The range of traditional applications of the Salvia herbs in domestic medicine seems to be endless: they have been used as a medication against perspiration and fever, as a carminative, spasmolytic, an antiseptic/bactericide, astringent, as gargles or mouthwashes against mouth, tongue and throat inflammation, as a wound healing agent, as a cure for skin and hair, against rheumatism and sexual debility, in treating mental and nervous conditions and as an insecticide [14,15,16,17]

Summary

Introduction

It is well known that reactive oxygen species (ROS) such as O2 (superoxide anion), H2O2(hydrogen peroxide), and OH (hydroxyl radical) are closely involved in various human diseases such as Alzheimer's disease, aging, cancer, inflammation, rheumatoid arthritis and atherosclerosis [1,2,3]. The ethyl acetate fraction of Salvia mirzayanii showed significant antioxidant activity in comparison with crude extract as well as the other fractions [18]. Antioxidant activity can be measured by radical scavenging activity and reducing power. The total amount of phenolic and flavonoids compounds from the ethyl acetate subfractions has been determined.

Results

Conclusion