Radical Scavenging and Antibacterial Activities of Ruta buxbaumii Poir. (Rutaceae) Growing in Raman Mountain-Batman

Abstract

The members of the Rutaceae genus have found a wide application area in traditional medicine in many countries from ancient times to today, they have been used for many purposes in Turkey. Ruta species have many pharmacological properties such as abortive treatment, as antirheumatic, hypoglycemic, ant-helmintic, antipyretic, antiepileptic, in the treatment of diabetes, epilepsy, vertigo, headache and eye dise-ases, in the elimination of intestinal worms and in poisoning. In this paper, it was aimed to determine the antimicrobial and radical scavenging activities of methanol (MeOH), Petroleum ether, EtOAc and ethanol (EtOH) extracts of wild Ruta buxbaumii Poir. Antimicrobial activities of extracts were evaluated using disc diffusion method. The extracts were also found to have microbe inhibiting activities. The antioxidant properties and activities were evaluated by using total phenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity. The results showed that while ethanol extract of Ruta buxbaumii was higher (54.10±0.13) than other extracts in terms of total phenolic substance content, met-hanol and ethanol extracts were found higher (47.52±0.19 and 46.86±0.16) than other extracts in terms of total flavonoid content, respectively. Also, radical scavenging activities such as 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) of extracts was investigated, and according to the solvent, it was revealed that MeOH extracts (73.6 %) of Ruta buxbaumii exhibited high activity. This study indicates that R. buxbaumii Poir. extracts could be used as a natural medical application in antimicrobial and antioxidant treatments.