Radical Scavenging Activity Combination of Sambiloto (Andrographis paniculata Nees.) and Patikan Kebo (Euphorbia hirta L.) Ethanolic Extracts on 2,2-Diphenyl-1-Picrylhidrazyl (DPPH)

Andayana Puspitasari Gani,Sitarina Widyarini,Sudibyo Martono,Suwidjiyo Pramono

doi:10.22146/mot.31600

Abstract

Research of radical scavenging activity of sambiloto (Andrographis paniculata Nees) and patikan kebo (Euphorbia hirta L) ethanolic extracts and their combination toward 2,2-Diphenyl-1-Picrylhidrazyl (DPPH) has conducted. Subjects of this experiment comprise sambiloto ethanolic extract, patikan kebo ethanolic extract, dechlorophyllated extract of sambiloto and combinations among those three. Dechlorophyllation by electrocoagulation method is done to reduce chlorophyll content thereby increasing the level of active compound in the sambiloto extract. The results of dechlorophyllation showed that only total flavonoid content in the extract of sambiloto increased, meanwhile the radical scavenging activity results showed that patikan kebo ethanolic extract has the lowest IC50 value (22,36 µg/mL) compared to sambiloto ethanolic extract (IC50 value 499,03 µg/mL). Extract combination may increase DPPH radical scavenging activity of the sambiloto extract (IC50 value 76,06 µg/mL) but not higher than that of patikan kebo single extract.

Highlights

Free radicals have been known to possess an important role in causing several chronic and degenerative diseases
Other compounds reported found in this plant are flavone class of flavonoid compounds (Jarukamjorn and Nemoto, 2008; Okhuarobo et al, 2014).Methanol and water extract of this plant are reported to have antioxidant activity determined based on DPPH radical capture method, Cupric ion method reducing antioxidant activity (CUPRAC), Ferric ion reducing antioxidant parameters (FRAP) and inhibition of lipid peroxidation
Determination of total flavonoid levels The total flavonoids levels in the ethanolic extract of patikan kebo (EEP), ethanolic extract of sambiloto (EES) and EEDS were determined by procedure carried out by Chang et al, (2002), which was modified

Summary

INTRODUCTION

Free radicals have been known to possess an important role in causing several chronic and degenerative diseases. Some plants have been proven to possess antioxidant activities; one of them is Sambiloto (Andrographis paniculata Nees.). Ethanolic extract of patikan kebo with active compounds β-amyrin can inhibit the function of iNOS protein and the induction of NO. It has the potential as an anti-inflammatory agent (Shih et al, 2010). Further observation in the effect of the combination of sambiloto’s ethanolic extract and dechlorophilized extract with patikan kebo herb’s ethanolic extract on free radical scavenging is carried out

METHODOLOGY

RESULTS AND DISCUSSION

CONCLUSION