Radiation treatment of bone hydatid disease

Abstract

Objective Explore the efficacy that radiation (6WV-X-ray) treat bone hydatid disease.Methods Establish seventy gerbils bone hydatid disease animal models which were randomly divided into four groups,one group was to be control group,the animal models in other three groups were given 40 Gy/5 times,50 Gy/5 times,60 Gy/5 times,6MV-X-ray positioning radiotherapy,3 months later(the end of radiotherapy),to observe the gerbils,general situation,to compare the mortality of scolex,protein and calcium concentration in capsule fluid,to observe the pathological changes of the horny layers and germinal layers,the varying of the bone cells in the irradiation areas and the ultrastructure of echinococcus granulosus cysts.Results Over the past 3 months,gerbils in each group has death cases,but the survival ones,general behaviors were normal.Among the groups,the difference of the mortality of scolex(such as Control group:22.6,40 Gy/5 times group:96.6,50 Gy/5 times group:131.5,60 Gy/5 times group:155.5) was statistically significant (P ＜ 0.01).Protein concentration (calcium ion concentration) in cyst fluid decrease (increase) with irradiation doses increase,The protein (calcium) concentrations are such as-the control group:1.0241 (2.8875),40 Gy/5 time group:0.7245 (3.0541),50 Gy/5 times group:0.6171 (3.5446) Gy to 60 Gy/5 times group:0.5058 (3.8241),which comgpared among each group there were significant differences(P ＜ 0.01).Histopathological results showed that echinococcus sac in the treatment groups showed different degrees of degeneration or necrosis,which compared with control group there were significant changing.Compared between 40 Gy and 50 Gy group,40 Gy and 60 Gy group,the pathological changes of echinoccus sac were significant difference (P ＜ 0.01),but between 50 Gy and 60 Gy group the pathological changes is not obvious (P ＞ 0.05).The bone cells of the irradiated groups have been varying degrees of degeneration or necrosis.By electron microscopy,with the irradiation doses increase,the horny layers and germinal layers have been varying degrees of damage.Conclusion Radiation therapy is effective for bone hydatid disease,but need to select the appropriate radiation dose according to the actual situation,to prevent the occurrence of irreversible osteonecrosis. Key words: Bone echinococcosis ; Model, animal; Radiotherapy