Radiation Synthesis of Selenium Nanoparticles Capped with β-Glucan and Its Immunostimulant Activity in Cytoxan-Induced Immunosuppressed Mice.

Nguyen Thi Dung,Tran Duc Trong,Le Quang Luan,Nguyen Trong Binh,Nguyen Thanh Vu,Tran Thi Le Minh

doi:10.3390/nano11092439

Nguyen Thi Dung, Tran Duc Trong + Show 4 more

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https://doi.org/10.3390/nano11092439

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Journal: Nanomaterials	Publication Date: Sep 18, 2021
Citations: 8	License type: CC BY 4.0

Affiliation: Nong Lam University Ho Chi Minh City

Abstract

Selenium nanoparticles (SeNPs) with diameters from 64.8 to 110.1 nm were successfully synthesized by γ-irradiation of solutions containing Se4+ and water-soluble yeast β-glucan. The size and size distribution of SeNPs were analyzed by dynamic light scattering (DLS). Analytical X-ray diffraction (XRD) pattern results confirmed the crystal structure of the Se nanoparticles and Fourier transform infrared (FTIR) spectroscopy revealed that β-glucan could interact with SeNPs through steric (Se…O) linkages leading to a homogeneous and translucent solution state for 60 days without any precipitates. In vivo tests in cytoxan-induced immunosuppressed mice revealed that the daily supplementation of SeNPs/β-glucan at concentrations of 6 mg per kg body weight of tested mice significantly stimulated the generation of cellular immune factors (white blood cells, neutrophil, lymphocyte, B cells, CD4+ cells, CD34+ cells and natural killer cells) and humoral immune indexes (IgM, IgG, TNF-α, IFN-γ and IL-2) in peripheral blood, bone marrow and spleen of the immunosuppressed mice. The obtained results indicated that radiation-synthesized SeNPs/β-glucan may be a candidate for further evaluation as an agent for the prevention of immunosuppression in chemotherapy.

Highlights

Selenium (Se) is an trace element essential for maintaining the health of mammalian animals due to its broad functions in biologic systems such as antioxidant, immune modulation, cancer prevention and antiviral activities [1,2,3]
It is reported that selenium nanoparticles (SeNPs) have higher antioxidant, immunostimulant, anticancer, antiviral and antibacterial effects compared to their ionic forms [12]
SeNPs have been already synthesized by γ-irradiation using oligochitosan as a stabilizer [16]

Summary

Introduction

Selenium (Se) is an trace element essential for maintaining the health of mammalian animals due to its broad functions in biologic systems such as antioxidant, immune modulation, cancer prevention and antiviral activities [1,2,3]. Selenide (Se−2 ) are commonly oxidation states of this metalloid element in the environment. Selenium nanoparticles (SeNPs) are prepared by reduction of higher oxidation states to the Se0 form [5,6,7]. It is reported that SeNPs have higher antioxidant, immunostimulant, anticancer, antiviral and antibacterial effects compared to their ionic forms [12]. The in vivo immunostimulatory effect of biogenic SeNPs on a 4T1 breast cancer model mice carried out by Nanomaterials 2021, 11, 2439.

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