Abstract
Triple sugar iron agar (TSI), a medium used for the identification of enteric bacteria, was sterilized by gamma radiation using radiation doses of 750–2000 krad. The radio-sterilized medium, slightly modified by increasing its Phenol Red content, performed well when tested with different enterobacteria-ceae and other gram negative bacteria. Growth, change of indicator reaction in slant and butt and formation of gas and H 2S were equal in irradiated and autoclaved TSI. Slants of irradiated TSI in stoppered plastic tubes kept their diagnostic properties during storage for at least 4 months. Gamma irradiation appears to be an attractive and economical method of sterilizing nutrient media in sealed tubes or other containers, avoiding the risk of contamination during processing.
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