Radiation sensitivity of endometrial carcinoma in vitro

Abstract

The role of radiation therapy is essential in the treatment of endometrial carcinoma. The present knowledge of the radiation sensitivity of endometrial carcinoma is mostly empirical and based on clinical trials. To study the inherent radiation sensitivity of endometrial carcinoma, we tested two long-established cell lines (RL95-2, KLE) and four new, low-passage cell lines (UM-EC-1, UM-EC-2, UM-EC-3, UT-EC-1) by using a 96-well plate clonogenic assay. This method has proved to be suitable for clonogenic studies of both squamous cell carcinomas (SCC) and adenocarcinomas in our recent works. Plating efficiencies of the cell lines tested varied from 0.005 to 0.45. Cells were irradiated in suspension with a 4-MeV linear accelerator at a dose rate of 2.0 Gy/min. Survival data were fitted by the linear quadratic function F = exp (−( αD + βD 2)). Radiation sensitivity was expressed as the area under the curve (AUC), equivalent to the mean inactivation dose. UM-EC-1 and UT-EC-1 were the most radiation-resistant cell lines tested (AUC > 1.6 Gy), while UM-EC-3 was the most sensitive (AUC = 1.0 Gy). The difference in radiation sensitivity between these cell lines was statistically significant ( P < 0.001). As a group, endometrial carcinoma cell lines were clearly more radiosensitive than most SCC lines of head and neck and vulva earlier tested by us. However, our results showed also great variance in the inherent radiation sensitivity between individual cell lines derived from endometrial carcinomas.