Abstract
Double-stranded DNA alone and with serum albumin in eight different weight ratios were irradiated with X-rays in phosphate buffer in air-free and aerated media. Double-strand breaks (dsb) were determined by electrophoresis. The oxygen enhancement ratio for dsb increased with increasing protein concentration from 0.8 to 3.0, presumably caused by the reaction of protein peroxyl radicals. In air-free media, serum albumin protects against radiation-induced double-strand breaking more effectively than in the presence of air, because a part of DNA radicals does not form dsb, but DNA–protein crosslinks.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
