Radiation-Induced Division and Mitotic Delay Studies in Green Alga (Oedogonium cardiacum)

Abstract

Studies on radiation induced division and mitotic delay have been made on Oedogonium cardiacum cells at different stages during the first cell cycle. The results obtained indicate that division and mitotic delay vary with the age of the cells at the time of irradiation. Further, both division and mitotic delay are maximum for cells irradiated in G2, moderate for cells irradiated in S, and a minimum for cells irradiated in G1. The division delay, measured by cell counting, increases linearly with dose after approximately 500 rads for cells in G1, S, and G2 and is 0.10, 0.19, and 0.33 minutes/rad, while mitotic delay, measured by the appearance of mitotic figures, is a linear function of dose for cells in G1, S, and G2 and is 0.10, 0.24, and 0.33 minutes/rad respectively.