Abstract
Abstract The traditional method of cloning formation is used to study the radiation effect of nuclear physics rays on hepatoma cells, and the results obtained are not reliable. Therefore, a new method of studying the radiation effect of nuclear physics rays on hepatoma cells is proposed. PCC method is used to study the radiation effect of γ-rays on hepatoma cells. Radiation effects of X-rays on hepatoma cells were studied by radionuclide formation assay, neutral comet electrophoresis and γH2AX focal detection. The results showed that the survival curves of HepG2 hepatoma cells irradiated by γ-rays were well fitted by linear squares. There was a linear relationship between the survival rate and irradiation dose of HepG2 hepatoma cells irradiated by γ-rays. HepG2 hepatoma cells showed strong tolerance to X-ray irradiation, and the positive rate of γH2AX cells reached 100% in each dose group only 0.5 h after X-ray irradiation.
Highlights
Cancer is one of the most lethal diseases
The results showed that double strand breaks (DSB) of HepG2 hepatoma cells induced by X-ray showed a time-and dose-dependent relationship, and the change of TM was most obvious at 4 hours after irradiation
The results showed that only 0.5 h after irradiation, the positive rate of γH2AX cells reached 100% in each dose group, and the high expression rate of γH2AX cells persisted until 4 h after irradiation; 24 h after irradiation, the positive rate of γH2AX cells in HepG2 hepatoma cells gradually decreased, but there was still signi cant di erence compared with the control group
Summary
Cancer is one of the most lethal diseases. Radiotherapy is currently one of the main means of liver cancer treatment. Radiobiologists study the radiation e ects of di erent kinds of nuclear physics rays on hepatocellular carci-. There are many methods to study the radiation e ect of γ-rays on hepatocellular carcinoma cells. Cloning is a common method to study the radiation effect of hepatocellular carcinoma cells. Premature chromosome agglutination (PCC) technique was used to study the radiation e ect of hepatocellular carcinoma (HCC), which took only 2 days from cell treatment to result statistics, but its application was limited. PCC technology has become a mature method to study radiationinduced primary chromosome damage in di erent cell cycle phases, especially in G2 phase [6]. There are few e ective methods to study the radiation e ect of X-ray on hepatoma cells, such as cloning, electrophoresis and so on [7]
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