Abstract
α 1-Acid glycoprotein (AGP) is an “acute phase protein” whose expression is altered in several human pathologies. Using antiserum from New Zealand white rabbits, a radial immunodiffusion assay for measuring AGP levels in rat plasma was developed operating in the range of 50–2500 μg/ml with high specificity. Standard curves were constructed (precipitin ring diameter 2 vs. μg/ml AGP) yielding highly linear plots (r=.98). The plasma concentration of AGP in spontaneously hypertensive (SHR) rats was double that of the normotensive Kyoto-Wistar (WKY) rats (208±10 vs. 118±5 μg/ml). AGP induction by turpentine resulted in a 14- and 26-fold increase in AGP levels in SHR and WKY rats, respectively. Induction of AGP by dexamethasone injection was examined in the SHR and WKY rat strains resulting in a 5- and 12-fold increase in AGP levels, respectively. AGP concentration in whole brain of rats was determined to be 12.7 ± 1.8 μg/g. AGP concentrations in SHR and WKY liver were also determined to be 159 ± 3 and148 ± 5 μg/g liver tissue.
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