Abstract
1. 1. Rabbit reticulocyte phosphofructokinase (EC 2.7.1.11) was purified by (NH 4) 2SO 4 precipitation, heat treatment and chromatography on Sepharose 4B. The specific activity of the enzyme was close to 170 units/mg. 2. 2. The comparison of reticulocyte phosphofructokinase and phosphofructokinase obtained from mature red cells has shwon the differences in the protective action of AMP and ATP, and in the inhibitory effect of citrate and the excess of ATP on phosphofructokinase activity. 3. 3. In reticulocyte hemolysate two activity bands were detected by means of polyacrylamide gel electrophoresis. In the mature red cell one of these activity bands was considerably diminished. 4. 4. It can be postulated that by cell maturation some modifications of the phosphofructokinase molecule may take place.
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