Abstract
Rab14 is a member of RAS oncogene family, and its dysfunction has been reported to be involved in various types of human cancer. However, its expression and function were still unclear in gastric cancer. The aim of this study was to investigate the function and mechanism of Rab14 in gastric cancer cell lines. Quantitative real-time PCR (qRT-PCR) was performed in 17 gastric adenocarcinoma tissues and 4 cell lines to detect the expression of Rab14. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT), colony formation and flow cytometry assays were employed to determine the proliferative ability, cell cycle transition and apoptosis in vitro in BGC-823 or SGC-7901 cells. Western blot was performed to investigate the pathways and mechanism of Rab14 regulation. In this study, we show that Rab14 presents a significant up-regulated expression among the paired tissue samples and cell lines in gastric cancer. When we overexpressed Rab14 in SGC-7901 cells or silenced Rab14 in BGC-823 cells, we found that Rab14 could modify cell growth, cell cycle or apoptosis, which accompanied with an obvious regulation of CCND1, CDK2 and BAX involving in AKT signaling pathway. In conclusion, this study provides a new evidence on that Rab14 functions as a novel tumor oncogene and could be a potential therapeutic target in gastric cancer.
Highlights
Gastric cancer (GC) is the most common cause of cancer-related death worldwide [1]
Rab14 is up regulated in GC tissue and cell lines
To determine whether Rab14 was a putative oncogene in GC, qRT–PCR analysis was performed to detect the expression levels of Rab14 in GC tumor and matched non-tumor tissues
Summary
Gastric cancer (GC) is the most common cause of cancer-related death worldwide [1]. China is one of the countries with a highest GC incidence rates accounting for over 40% of all GC cases worldwide [2, 3]. The tumorigenesis of GC is a multistep process, which results from. Rab Act as an Oncogene in Gastric Cancer design, data collection and analysis, decision to publish, or preparation of the manuscript
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