Abstract
Here, we report a novel virulent P2-like bacteriophage, R18C, isolated from rabbit faeces, which, in addition to Escherichia coli K-12 strains, was able to be propagated on Citrobacter rodentium strain ICC169 and a range of Shigella sonnei strains with high efficiency of plating (EOP). It represents the first lytic bacteriophage originating from rabbit and the first infectious P2-like phage of animal origin. In the three characteristic moron-containing regions of P2-like phages, R18C contains genes with unknown function that have so far only been found in cryptic P2-like prophages.
Highlights
We report the characterization of bacteriophage R18C isolated from rabbit faeces (kindly provided by László Makrai, University of Veterinary Medicine, Budapest, Hungary), which proved to be a new member of viable P2-like viruses
We report the characterization of bacteriophage R18C isolated from rabbit faeces, which proved to be a new member of viable P2-like viruses
The cell-free supernatant of the culture was spotted on layered soft agar plates containing E. coli K-12 strain MG1655 [4]
Summary
We report the characterization of bacteriophage R18C isolated from rabbit faeces (kindly provided by László Makrai, University of Veterinary Medicine, Budapest, Hungary), which proved to be a new member of viable P2-like viruses. The host spectrum and efficiency of plating (EOP) were assessed by spot assay on layered soft agar plates containing the respective strains according to standard protocols described in detail by Tóth et al [5].
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