Abstract
Quorum sensing is a unique bacterial communication system which permits bacteria to synchronize their behaviour in accordance with the population density. The operation of this communication network involves the use of diffusible autoinducer molecules, termed N-acylhomoserine lactones (AHLs). Serratia spp. are well known for their use of quorum sensing to regulate the expression of various genes. In this study, we aimed to characterized the AHL production of a bacterium designated as strain RB-25 isolated from a former domestic waste landfill site. It was identified as Serratia fonticola using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis and this was confirmed by 16S ribosomal DNA sequencing. High resolution triple quadrupole liquid chromatography-mass spectrometry analysis of S. fonticola strain RB-25 spent culture supernatant indicated the existence of three AHLs namely: N-butyryl-L-homoserine lactone (C4-HSL), N-hexanoyl-L-homoserine lactone (C6-HSL) and N-(3-oxohexanoyl) homoserine-lactone (3-oxo-C6 HSL). This is the first report of the production of these AHLs in S. fonticola.
Highlights
Serratia spp. are well known for the production of prodigiosin [1]
Identification of the isolate was done by using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis and sequence analysis of 16S ribosomal DNA
Bacterial isolates were streaked in several passages in order to obtain single, pure colonies and the bacteria were examined for both acylhomoserine lactones (AHLs) production and degradation using the two types of biosensors
Summary
Serratia spp. are well known for the production of prodigiosin [1]. As part of the Enterobacteriaceae family, Serratia spp. are frequently reported to be the cause of food spoilage [1]. QS is achieved by bacteria via production of and response upon detection to various QS signaling molecules termed autoinducers These signaling mechanisms enable the bacterial population to maintain the cell density within individual species as well as among other species in the surrounding environment via detection of accumulated concentrations of exogenous signaling molecules, and subsequently allow regulation of the expression of diverse beneficial genes. Identification of the isolate was done by using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis and sequence analysis of 16S ribosomal DNA (rDNA). Its AHL profile was confirmed unequivocally by using high resolution quadrupole mass spectrometry The former landfill site was selected as the sampling site as there is limited documentation about the microbial population with QS properties in this unique environment
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