Abstract
Cell-cell communication is also known as quorum sensing (QS) that happens in the bacterial cells with the aim to regulate their genes expression in response to increased cell density. In this study, a bacterium (L8A) isolated from dental plaque biofilm was identified as Citrobacter amalonaticus by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Its N-acylhomoserine-lactone (AHL) production was screened by using two types of AHL biosensors namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Citrobacter amalonaticus strain L8A was identified and confirmed producing numerous types of AHL namely N-butyryl-L-homoserine lactone (C4-HSL), N-hexanoyl-L-homoserine lactone (C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL) and N-hexadecanoyl-L-homoserine lactone (C16-HSL). We performed the whole genome sequence analysis of this oral isolate where its genome sequence reveals the presence of QS signal synthase gene and our work will pave the ways to study the function of the related QS genes in this bacterium.
Highlights
In the past few generations, dental scientists and microbiologists as well as microbial ecologists have researched on the identification of oral microbes which have led to oral diseases
The aim of this study is to understand the occurrence of quorum sensing (QS) in C. amalonaticus isolated from dental plaque by studying the acylated-L-homoserine lactones (AHLs) production profile and insights gained from the annotated genomic features of C. amalonaticus strain L8A
Using MALDI-TOF mass spectrometry, strain L8A was identified at species level with score value of 2.390 and it was best matched to Citrobacter amalonaticus
Summary
In the past few generations, dental scientists and microbiologists as well as microbial ecologists have researched on the identification of oral microbes which have led to oral diseases. Dental plaque forms naturally on the teeth and aids in colonization of exogenous species, which disturbs the microbial homeostasis within oral cavity and becomes predisposed sites to the disease[2]. This diseased dental plaque influences the changes in environmental conditions and plays a vital role in the site specific diseases such as dental caries, gingivitis and periodontitis[3]. Previous researches show that a minimum number of 800 bacterial species are present in the oral cavity[5,6] out of which total estimate of 415 species and 68 unseen species were reported from sub-gingival dental plaque[5]. The aim of this study is to understand the occurrence of QS in C. amalonaticus isolated from dental plaque by studying the AHL production profile and insights gained from the annotated genomic features of C. amalonaticus strain L8A
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