Quick Release of Hydrogen Peroxide from Carbamide Peroxide Promotes Apoptosis of A549 Lung Cancer Cells

Abstract

AbstractLung cancer is one of the malignant tumors with the leading morbidity and mortality worldwide. H2O2 is an important oxidant, and lethal to tumor cells. The present study aimed to explore new higher‐H2O2‐content and faster‐H2O2‐release rate carbamide peroxide (CP) compounds for treatment of lung cancer. The CP added with benzoic acid termed CP−B was applied in the research. Fenton reaction was used to determine the content and rate of H2O2 release by degrading organic small molecule dyes. The results showed that CP−B could be decolorized 200 mg/L RhB solution within 60 s. The phenotype was analyzed by vitro experiment the effects of the CP compound on A549 lung cancer cell line. The results showed that CP−B had a significant effect on pro‐apoptosis, anti‐proliferation and anti‐migration of A549 cells. Using RNA seq, it was found that CP−B might up‐regulate the INS‐IGF2 gene and it was closely related to the PI3 K/AKT signaling pathway. Finally, in vivo experiments indicated that CP−B has extremely low side effects and potentially tumor treatment capabilities.